CodeLink SNP

About SNP

CodeLink™ SNP is a bioarray system solution for broadbased SNP genotyping and pharmacogenetic profiling. Using the power of highly multiplexed assay processing, proprietary in-silico primer probe design, and bioarray-based fluorescence detection, the CodeLink SNP system enables the efficient and cost-effective measurement of large numbers of SNPs per sample and offers an unparalleled opportunity to uncover novel relationships between genotype and phenotype.

The CodeLink SNP Bioarray System

At the heart of CodeLink SNP is a highly multiplexed assay involving i) PCR amplification and amplicon fragmentation ii) surface-based enzymatic allele discrimination, and iii) array-based fluorescence labelling and detection.



PCR design and primer plates

PCR design is an important component of the CodeLink SNP system because the performance of a genotyping technology is determined in large part by the specificity of allele amplification. Uniplex PCR is sufficient (and occasionally essential) for some applications such as candidate gene studies while multiplex PCR is an important feature of disease linkage and association studies where large numbers of SNPs are to be analyzed. For most applications, 8-, 16-, 24-, and 32-plex PCR reactions are routine with CodeLink and multiplexing as high as 96-plex has been achieved. High throughput allele amplification can be performed with off-the-shelf robotics systems.

SNP system software

Upon fluorescence scanning of the bioarrays, the CodeLink System software automatically grids the image, performs background subtraction, and calculates signal intensities at each spot. Genotyping calls are achieved with a defined threshold-based automated calling tool within the system software. Genotyping reports are generated within the reporting function of the system software.