(384well.pdf - 28 Kb)

Scintillation proximity assay (SPA) is a homogeneous assay technology that has been successfully applied to many drug screening applications. By coupling a range of specific molecules to SPA particles, receptor binding assays, enzyme assays, molecular interaction assays and radioimmunoassays have been established. SPA is applicable to high throughput screening and can be adapted for automation. Assays are routinely performed in 96 well microplates (MP), but many users are converting to utilization of 384 well plates. The potential advantages of assay miniaturization are as follows: reduction in plate usage, as one 384 well plate is equivalent to four 96 well plates. reduction in reagent usage, for example 200 ml assay can be reduced to 50 ml volume increased throughput, particularly if the process is automated.